Laemmli gel loading buffer (4x)
4ml of 1M Tris-HCl pH 6.8
2g SDS
4ml Bromophenol blue 2%
8ml glyrerol
0.6 ml Beta-mercaptoethanol
Mix at RT, heat up to ~ 80 C for 2-5 mins and leave at RT. Storage: RT
(Recipe from Thierry)
Laemmli gel loading buffer (4x)
4ml of 1M Tris-HCl pH 6.8
2g SDS
4ml Bromophenol blue 2%
8ml glyrerol
0.6 ml Beta-mercaptoethanol
Mix at RT, heat up to ~ 80 C for 2-5 mins and leave at RT. Storage: RT
(Recipe from Thierry)